PNPLA3 Knockout cell line (Hep G2)

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PNPLA3 Knockout cell line (Hep G2) Order

PNPLA3 Knockout cell line (Hep G2)
Catalog#
YKO-HT1868
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PNPLA3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PNPLA3 Knockout cell line (Hep G2)
Species
Human
Gene Name
PNPLA3
Gene ID
80339
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript PNPLA3-201)
Validation: PCR+Sanger Sequencing
PNPLA3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PNPLA3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PNPLA3 gene knockout PNPLA3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PNPLA3 Knockout cell line (Hep G2)
PNPLA3 Knockout cell line (Hep G2)
Catalog#
YKO-HT1868
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PNPLA3 Knockout cell line (HeLa)

    PNPLA3 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92177
    YKO-XN92177(HeLa)
  • PNPLA3 Knockout cell line (HCT 116)

    PNPLA3 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92176
    YKO-XN92176(HCT 116)
  • PNPLA3 Knockout cell line (HEK293)

    PNPLA3 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92175
    YKO-XN92175(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PNPLA3 Knockout cell line (HeLa)

    PNPLA3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92177
      inquiry   More details
  • PNPLA3 Knockout cell line (HCT 116)

    PNPLA3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92176
      inquiry   More details
  • PNPLA3 Knockout cell line (HEK293)

    PNPLA3 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92175
      inquiry   More details

PNPLA3 Knockout cell line (Hep G2) Order

PNPLA3 Knockout cell line (Hep G2)
Catalog#
YKO-HT1868
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PNPLA3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PNPLA3 Knockout cell line (Hep G2)
Species
Human
Gene Name
PNPLA3
Gene ID
80339
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript PNPLA3-201)
Validation: PCR+Sanger Sequencing
PNPLA3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PNPLA3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PNPLA3 gene knockout PNPLA3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PNPLA3 Knockout cell line (Hep G2)
PNPLA3 Knockout cell line (Hep G2)
Catalog#
YKO-HT1868
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PNPLA3 Knockout cell line (HeLa)

    PNPLA3 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92177
    YKO-XN92177(HeLa)
  • PNPLA3 Knockout cell line (HCT 116)

    PNPLA3 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92176
    YKO-XN92176(HCT 116)
  • PNPLA3 Knockout cell line (HEK293)

    PNPLA3 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92175
    YKO-XN92175(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PNPLA3 Knockout cell line (HeLa)

    PNPLA3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92177
      inquiry   More details
  • PNPLA3 Knockout cell line (HCT 116)

    PNPLA3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92176
      inquiry   More details
  • PNPLA3 Knockout cell line (HEK293)

    PNPLA3 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: PNPLA3
    Gene ID: 80339
    Size: 1*10^6
    Catalog#: YKO-XN92175
      inquiry   More details

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